Novel Automated Platforms for CRISPR-Engineered Cell Lines and Synthetic sgRNA for Primary Cell and Screening Applications

When and Where

Date: Thursday, November 29, 2018 - Thursday, November 29, 2018 (1 day)
Price: Free Location: Ontario Institute for Cancer Research (661 University Ave)
Room 5-20/21 Toronto, ON, M5G 1M1 View map »

The seminar offers a fantastic opportunity to learn from the best in the CRISPR gene editing field, which has gained importance as a research tool, in drug discovery, screening, and therapeutic development. 

Synthego is a world leading provider of genome engineering solutions. Its flagship product, CRISPRevolution, is well recognized for its immediate impact on life sciences and it is poised to transform the industry by making the application of CRISPR simpler, faster, and more valuable to researchers.

Registration is free, but space is limited.

Pathologists and Researchers who are interested in being part of the OMPRN:

3rd Annual NanoString R&D Consortium Day

When and Where

Date: Monday, September 24, 2018 - Monday, September 24, 2018 (1 day)
Price: free Location:

Join NanoString® and Ontario Scientists as we discuss the latest NanoString advances in both life science research and clinical diagnostic applications. In addition to local users presenting research data focused on gene expression, NanoString will discuss new and exciting applications and technologies like our IO360™ and Breast Cancer 360™ Panels specifically designed for translational research, Digital Spatial Profiling (DSP), PlexSet™ high-throughput assays and Hyb & Seq™.

September 24, 12-4pm at Peter Gilgan Centre for Research and Learning

Pathologists and Researchers who are interested in being part of the OMPRN:

Governance - Dr. Michelle Downes

Governance - Dr. Tracy Stockley

OICR Biolabs: PrimeFlow RNA Assay for simultaneous detection of RNA and Protein

When and Where

Date: Thursday, November 8, 2018 - Thursday, November 8, 2018 (1 day)
Price: free Location:

Registration Contact

Dr. Vanya Peltekova
Send Email »

This presentation will provide an overview of the PrimeFlow RNA Assay, which expands the capability of flow cytometry to also measure gene expression. By combining PrimeFlow with traditional immunolabeling of both cell-surface and intracellular proteins, researchers can now reveal the dynamics of both RNA transcription and protein expression patterns at the single cell level. Included in this discussion will be an introduction to branched DNA technology, assay workflow, and specific applications from the published literature.

Thu, November 8, 2018 2:00 PM – 3:00 PM

Pathologists and Researchers who are interested in being part of the OMPRN:

OICR Biolabs: Three-Dimensional Organoid Cultures as in vitro Models for Cancer Research

When and Where

Date: Wednesday, October 24, 2018 - Wednesday, October 24, 2018 (1 day)
Price: free Location:

Registration Contact

Dr. Vanya Peltekova
Send Email »

Researchers that study human diseases, including cancer, often rely on animal models or cell lines that grow in culture to investigate the mechanisms that underlie the disorder. While there are benefits to using animals and cell lines, there are also many obvious problems. To address this issue, scientists have found a way to create miniature models of human organs in the lab, called organoids, which can grow in a dish as 3D structures. Organoids have become useful tools with many applications from disease modeling and pharmacological screening to regenerative medicine and cancer research.

Wed, October 24, 2018 2:00 PM – 3:00 PM

Pathologists and Researchers who are interested in being part of the OMPRN:

OICR Biolabs: Introduction to Multiphoton Microscopy and Fluorescence Lifetime

When and Where

Date: Tuesday, October 16, 2018 - Tuesday, October 16, 2018 (1 day)
Price: free Location:

Registration Contact

Dr. Vanya Peltekova
Send Email »

A confocal microscope based on visible light can leave many details invisible. Often the image you need is hidden deep within tissue, which not only scatters light but is damaged by it too. The excellent optical design of the Leica TCS SP8 DIVE/FALCON system combines use of long wavelengths of fully integrated infrared excitation lasers, the high efficiency of HyD detectors, and an infrared-optimized optical transmission. These components allow deeper penetration into the tissue with super-sensitivity to uncover the finest details of cellular and subcellular processes. This system is the best for thick organ sections, intact organs or entire live model organisms.

Tue, October 16, 2018 2:00 PM – 3:00 PM at OICR.

Pathologists and Researchers who are interested in being part of the OMPRN:

OICR Biolabs: The Moffitt Cancer Center approach to pathology diagnostics

When and Where

Date: Tuesday, October 16, 2018 - Tuesday, October 16, 2018 (1 day)
Price: free Location:

Registration Contact

Dr. Vanya Peltekova
Send Email »

Dr. Anthony Magliocco has worked to establish the Moffitt Morsani Molecular Laboratories and a new CLIA microscopic digital image analysis facility. These new laboratory programs bring innovative CLIA grade testing services to the patients and physicians supporting the Personalized Medicine Program. Advanced testing services include next-generation sequencing, circulating tumour cell analysis, cell-free nucleic acid analysis and complex multiplex analytical tissue imaging.

Tue, October 16, 2018 11:00 AM – 12:00 PM at OICR

Pathologists and Researchers who are interested in being part of the OMPRN:

OICR Biolabs: Global Transcriptome Profiling for Better Biomarkers

When and Where

Date: Wednesday, September 5, 2018 - Wednesday, September 5, 2018 (1 day)
Price: free Location:

Registration Contact

Dr. Vanya Peltekova
Send Email »

The number of known transcribed genes has expanded rapidly in recent years, providing more sources for actionable biomarkers, such as transcript variants and lncRNA, that can be used for clinical utility and advancing our understanding of disease mechanisms. Such biomarkers can be missed if careful consideration of techniques and the samples used is not thorough, leading to irreproducible signatures and wasted time and money.

Wed, September 5, 2018 3:00 PM – 4:00 PM at OICR

Pathologists and Researchers who are interested in being part of the OMPRN:

Pathology Image Analysis Training workshop

When and Where

Date: Friday, July 20, 2018 - Friday, July 20, 2018 (1 day)
Price: $175-245 Location: 101 College Street, 7th floor, MaRS Building, Princess Margaret Cancer Research Tower – East
Toronto, ON, M5G 1L7 View map »

Registration Contact

Dr. Trevor McKee
Send Email »

TissueStudio Pathology Analysis Workshop, STTARR Facility, 20th July 2018, 10am-4pm

Announcing the second Pathology Image Analysis Training workshop, to happen on 20th July 2018 at the STTARR Innovation Centre in the MaRS Complex East Tower (Princess Margaret Research Tower). This STTARR workshop is designed to explain how to generate quantitative per-cell statistics from brightfield or immunofluorescent stained digital pathology slides or images. The image analysis core of STTARR has many years of experience in utilizing Definiens TissueStudio platform, and custom-built post-processing analysis tools, that are offered on a fee-for-service basis to any users in the Toronto area wishing to perform image analysis.

Definiens is a market leader in digital tissue pathology analysis, particularly for complex immune-oncology related biomarkers. It is used by many pharmaceutical companies for biomarker discovery to identify meaningful companion diagnostics. TissueStudio can analyze brightfield, immunofluorescence, or imaging mass cytometry data, in a reproducible, batch format with built-in quality control/manual correction. In addition to covering usage of this software, we will review general principles for effective analysis of digital pathology images, and provide hands-on training for researchers wishing to perform quantitative analysis on their images.

Target Audience: Graduate students, technicians, research associates, PI's, and postdocs requiring image analysis on whole slide images or images from high content screening tools. This course will provide a solid foundation for quantitative evaluation of immunostained slides, helping to optimize your image analysis methods for the most reliable data from your images.

Date: REVISED DATE! 20th July 2018, 10:00am – 4:00pm

Location: STTARR Innovation Centre, 101 College Street, 7th floor, MaRS Building, Princess Margaret Cancer Research Tower – East, Toronto, ON M5G 1L7

Draft Agenda:

10am-2pm Presentations by STTARR Image Analysis staff on analysis pipeline methods

12-1pm Lunch (provided)

2-4pm Hands-on training/demo on Definiens Tissue Studio image analysis software

4pm Final questions / conclusion

Pathologists and Researchers who are interested in being part of the OMPRN:

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