London Regional Cell and In Vitro Molecular Imaging (Robarts Research Institute)

London

London Regional Cell and In Vitro Molecular Imaging (Robarts Research Institute)

About the Service Provider

We are the principal Confocal Imaging Facility in the City of London that was first established with MRC funding in 1999 by Dr Ferguson and built upon  with continuous rounds of CFI/ORF funding. Our mission is to provide the best affordable, high quality, accessible, and user-friendly laser scanning confocal microscopy services for all users in Southwestern Ontario. The two sites of our Facility are located at Robarts Institute, where you can find image analysis workstations, a cell culture facility and 6 imaging systems.

We have 3 Zeiss LSM 510 Meta microscopes, one equipped with a Confocor2 for Fluorescence Correlation Spectroscopy (FCS) and another with a femtosecond laser for multiphoton microscopy. We also have an Olympus FV1000 Confocal Microscope with enclosed chamber for live cell imaging, a Leica TCS SP5 II confocal microscope and a Leica AM TIRF MC that is specialized system for imaging cell surface events. We recently acquired a Ground State Depletion Super resolution system from Leica Microsystems and incorporated a Zeiss Apotome microscope to our collection! In addition, the facility offers Imaris Software and Matlab for imaging analysis and a dedicated system for radiometric calcium imaging.

We provide researchers with diversity and flexibility with respect to experimental approaches and techniques for molecular imaging. Besides, we offer a complete training and assistance.

The facility is available to all researchers at Robarts, UWO and entire London regional research community.

Services offered:

  • Widefield fluorescence microscopy
  • Laser scanning confocal microscopy
  • Multiphoton microscopy
  • Fluorescence correlation spectroscopy (FCS)
  • Total internal reflection fluorescence microscopy (TIRF)
  • Apotome - structured illumination microscopy
  • FRET
  • FRAP
  • Emission fingerprinting
  • Photoconversion

Equipment available:

  • Leica AM TIRF MC
  • Leica SR GSD super-resolution microscope
  • Leica TCS SP5
  • Olympus FLUOVIEW FV1000
  • PTI DeltaRAMV
  • ZEISS ApoTome
  • ZEISS LSM 510 META
  • ZEISS LSM 510 META ConfoCor2
  • ZEISS LSM 510 META NLO

Location

1151 Richmond Street North
Robarts Research Institute, Rooms 3260 and 3281 London, ON, N6A 5B7

Point of Contact

For further information about this service provider, please contact:
Flavio Beraldo


Available Services

Pre-analytical

  • Consultation
  • Project Design

Analytical

  • Fluorescence Microscopy

Post-analytical

  • Image analysis
Pathologists and Researchers who are interested in being part of the OMPRN: